BS EN 14105:2020 pdf free

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BS EN 14105:2020 pdf free.Fat and oil derivatives—Fatty Acid Methyl Esters (FAME]一Determination of free and total glycerol and mono-, di-, triglyceride contents.
BS EN 14105 This document specifies a method to determine the free glycerol and residual mono-, di- and triglyceride contents in fatty acid methyl esters (FAME). The total glycerol content is then calculated from the obtained results.Under the conditions described, the quantification limits are 0,001 % (m/m) for free glycerol, 0,10 % (m/m) for all glycerides (mono-, di- and tri-). This method is suitable for FAME prepared from rapeseed, sunflower, soybean, palm, animal oils and fats and mixture of them. It is not suitable for FAME produced from or containing coconut and palm kernel oils derivatives because of overlapping of different glyceride peaks.NOTE 1 For the purposes of this document, the term“% (m/m)” is used to represent the mass fraction.NOTE 2 Under the common EN 14105 GC conditions squalene can coelute with alpha glycerol monostearate. If the presence of squalene is suspected, EN 17057 can be used to discriminate between squalene and glycerol monostearate. WARNING一The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to application of the standard, and fulfil statutory and regulatory requirements for this purpose.Using a precision pipette (6.9), add 150 ul of MSTFA (5.1) to each of the four calibration solutions (7.5), close hermetically the vials and shake vigorously. Store 15 min at room temperature. Then open the vial and add 8 ml of n-heptane (5.4) using a graduated cylinder (6.10]. Close the vial hermetically again. Analyse 1 μl of each reaction mixture by gas chromatography e.g. under the conditions indicated in 9.1,using only the first part of the temperature programme, stopping the analysis when the temperature of 230 °C has been reached. Samples are stable for some hours after derivatisation. BS EN 14105 pdf downlaod.

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