BS EN ISO 16140-5:2020 pdf free.Microbiology of the food chain一Method validation.
The aim of the interlaboratory study is to compare the performance of the reference method to the alternative method in terms of the RLOD obtained by different laboratories. The results of the same set of samples, examined under reproducibility conditions, are compared with pre-set criteria for the
acceptable difference between the reference method and the alternative method. W herever possible, the study conditions should reflect the normal variation between laboratories. The interlaboratory study is planned by the organizing laboratory. The organizing laboratory prepares the samples and a data sheet for the recording of all experimental data and critical experimental conditions used by each laboratory. Each laboratory shall demonstrate its competence in the use of the alternative and the reference method in accordance with ISO 7218 prior to participating in the study. Technicians involved in the preparation of the samples used in the interlaboratory study shall not take part in the testing of the samples in the interlaboratory study.In cases where different enrichment protocols for the alternative method exist, the most challenging enrichment protocol shall be selected, e.g. the protocol having the shortest incubation time or the most selective enrichment conditions. The selected [food) type shall be relevant for the chosen enrichment protocol. The {food) item, which is used to prepare the test samples, should contain natural background microbiota. The selected [food) item shall be artificially contaminated with the target organism. The protocol for inoculation of the samples shall be appropriate for the selected [food) item. Samples shall be prepared by the organizing laboratory to ensure homogeneity between and within samples using preparation protocols contained in ISO 16140-2:2016, Annex C. In general, liquid samples [compared to solid samples) give greater assurance of homogeneity. The samples should be shown to be homogeneous by the organizing laboratory. Homogeneity tests and criteria for acceptance are described in ISO 22117.BS EN ISO 16140-5 pdf download.