BS EN ISO 20418-3:2020 pdf free.Textiles一Qualitative and quantitative proteomic analysis of some animal hair fibres.
8.1 Sampling The general requirement is that the test specimen shall be representative for the lot of material from which it is taken. The method to obtain a fibre test specimen differs depending on the sample form. The terms relating to sampling for the various types of samples shall be in accordance with ISO 1833-1. 8.2 Preliminary identification The preliminary qualitative analysis of the animal hair fibre shall be carried out based on their morphology, which is determined using light microscopy, according to ISO 17751 (all parts), after removal of non-animal fibre.  8.3 Wash for degreasing 8.3.1 Reflux 1 g of the fibres in a recovery flask (7.11) on a heating mantle (7.1) with 200 ml of acetone (6.1) for 30 min. This washing step may be omitted in the case of clean samples. Quantity of the fibres can be changed. 8.3.2 Take the degreased fibres out of the recovery flask and dry them in the air. Alternatively, the sample preparation method of ISO 20418-1[5] can be used.8.4 Powderization of fibres Crush the dried fibre sample (8.3.2) using a mill (7.2) to get a fine powder with an average length of 100 μum or less by checking under the microscope and mix thoroughly for securing representative sampling of the fibres.  8.5 Trypsin digestion 8.5.1 Weigh about 10 mg of the crushed sample and place it into a microtube. If more than 10 mg of the sample is used, increase the volumes of the NH4HCO3 solution in 8.5.2 and Trypsin solution in 8.5.3 proportionally. 8.5.2 Add 300 μl of the NHqHCO3 solution (6.3) and vortex for 10 min to 30 min. 8.5.3 Add 10 μl of the Trypsin solution (6.2) to the sample and incubate at 37 °C for 20 h to24 h. 8.5.4 Centrifuge the tryptic solution for 3 min using the centrifugal evaporator. Filter the supernatant through a membrane filter to remove residual fibres. NOTE Centrifugal filter, syringe filter or other means of filtration can be used.8.5.5 Transfer the solution to an LC vial, then dry it using the centrifugal evaporator. If the LC vial  does not fit in the dryer, the solution can be dried in other types of container such as a microtube. The sample is transferred to an LC vial after dissolution. Alternatively, a freeze dryer or nitrogen flux can be used as the drying method, instead of the centrifugal evaporator.BS EN ISO 20418-3 pdf download.